The effect of different energy drinks on Daphnia magna heart rate eggs production, mortality, and activity level

1. Give a brief introduction (1-2 short paragraphs). State your purpose in doing the study. How does this work relate to the real world? What was the main question(s) you were attempting to answer? [10 pts]

2. Methods: Give a brief description of how you conducted your study. What were the variables measured? How did you measure them? How many subjects were included in your study? What sort of controls did you implement to avoid/prevent bias and sampling error? [20 pts]
3. State your population of interest (in other words, what was the population about which you wanted information?). Do you think your sample obtained was representative of the whole population (in other words, what was the population for which these statistical analyses will actually apply)? If you were doing this analysis in the “real” world, how would you ideally have collected your sample? [10 pts]
4. Describe and display the data.
   a. For two of your qualitative variables: [15 pts]
5. Make a frequency table showing the number of individuals in each category.
6. Display these data graphically using a bar chart. Embed this graph in the text of your write-up.
7. You might have a qualitative variable with only two levels (e.g. male and female). In such a case, your frequency tables and graphs will not be very interesting. That’s ok.

b. For two of your quantitative variables: [15 pts]

1. Calculate the mean, median, mode, range, standard deviation, Q1, Q3, and IQR.
2. Using Excel or SPSS, draw a histogram with between 5 and 12 bins. Embed this graph in the text of your write-up. Discuss your findings by describing the shape of the distribution and pointing out any interesting or unusual features.
3. Analyze the data using Excel and/or SPSS. [60 points total, you are probably running 2 or 3 hypothesis tests, the points will be split evenly across all the tests you run]
   a. Conduct a hypothesis test of your choice.
   i. Show the hypotheses
   ii. Perform the appropriate hypothesis test (remember assumptions!)
   iii. Determine the p-value and make a decision. Use alpha = 0.05.
   iv. State your biological conclusions with properly reported p values.
   b. Conduct a different hypothesis test remember you have 2 quantitative and 2 qualitative variables, ALL variables need to be used at least once in a hypothesis test. Repeat steps i-iv for this test.