The amino acid composition

Which method would you use and why?
3) In developing this lab, we had to make a decision on what protease to use to
separate our protein of interest from the GST tag. We had a number of proteases
to choose from. Copy and paste the amino acids sequence provided
(GST+ protein X) into the PeptideCutter tool window
(http://ca.expasy.org/tools/peptidecutter/) and look at the following enzymes for
cleavage patterns: trypsin and thrombin. Print out your results and answer why you
think we chose thrombin over trypsin. Also indicate where thrombin cleaves.
4) Copy and paste the amino acid sequence of the shorter peptide segment
(post-cleavage with thrombin) in Protparam window (http://ca.expasy.org/tools
[protparam.html)
to gather the following information:
a) the amino acid composition in percentage
b) the theoretical pl
c) the estimated molecular weight
d) the total number of negatively charged residues
e) the total number of positively charged residues
) the atomic composition (number of CH,N,O,S)
g) total number of atoms
h) the aliphatic index
5) Can you identify this protein using this program? (Use Protein BLAST at the
National Center for Biotechnology Information at
https://blast.ncbi.nlm.nih.gov/Blast.cgi
6) Attach all of the obtained data in the Appendices section of your lab report.
Important note on using bioinformatics web-based data in your lab report: you
must provide correct and complete references for the programs that you used to obtain
any (and all) data for your lab report. Complete references for the programs, such as
Protparam, Protein BLAST, etc. are provided on the websites these programs run from. It
is incorrect to provide an Internet (weblink) reference only. For example, click on the
References/Documentation link in the main ProtParam webpage (located above the data
entry window) to view the original references for this program. You will lose marks on
your lab report 4 if you do not provide original references for the web-based programs
you used to obtain your data. Attach all data obtained to your lab report in the
Appendices section.