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When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on?
Distinguish between a pure culture and a mixed culture.
Define a bacterial colony. List four characteristics by which bacterial colonies may
be distinguished.
Why should a Petri dish not be left open for any extended period?
Why does the streaking method you used to inoculate your plates result in isolated colonies?
Exercise 5: Pour plate and streaking technique to obtain pure cultures
Discuss the relative convenience of pour- and streak-plate techniques in culturing clinical
specimens.
How do you decide which colonies should be picked from a plate culture of a mixed flora?
Why is it necessary to make pure subcultures of organisms grown from clinical specimens?
What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
When more than one colony type appears in pure culture, what are the most likely sources of
extraneous contamination?
Exercise 3: Primary media for isolation of microorganisms
Define a differential medium and discuss its purpose.
Define a selective medium and describe its uses.
Why is MacConkey agar selective as well as differential?
Why is blood agar useful as a primary isolation medium?
What is the major difference between Modified Thayer-Martin (MTM) and chocolate agar? When
would you use MTM rather than chocolate agar