Osmolytes and stabilization

Osmolytes and stabilization Project description 1-abstract summary and goal of our project ( the goal is we try stable the silk by use different concentration of water and osmolytes insted use HFIP 2-INTERODUICTION about silk and osmolytes ( betaine , TMANO, and sarcosine) also write about CD 3-expermental procedures and put all tables 4-result put all graphs 5- discussion all the result 6-concluion summary and applications of betaine and TMANO and sarcosine ill give you the attached AND MAKE THE REPORT FROM IT Experimental Procedure • Make an 4.4M stock solution (concentration) by adding 5.5g of TMANO( 111.14 g/mol,solubility=6M) to 10 ml of water, so the TMANO would dissolve in solution. • prepare the water and HFIP of silk mixtures. • Add the TMANO stock solution in the specified concentration to the the water and HFIP mixtures. • add 20mM of synthetic 15- repeat of silk with water to each eppendorf tubes fisher. • check the room temperature before using Circular Dichroism to collect date.