Method the new method

Method the new method The method of collection of immature oocytes can affect mitochondrial morphology and function : Follicle containing immature oocytes can be collected from ovaroan tissue by Mechanical disaggregation (use forceps and seisors 2. Enzyme digestion of the extra cellular matrix and connective tissue that the follicles embedded in - collagenase - DNAse - Libarase - tryse 3. A combination of mitochondrial and enzyme disagrregation Collect ovaries from naturally cycling mice culled in routine anthiel hwse procedures 2. Dissect into rephiate small sections 3. Distribute to 4% paraformaldehyde fixation H+E histology to quantity follicule Mechanical disaggregation Collagenase concentration 1, 2 & 3 Trypsin concentration 1, 2 & 3 Selected cllagenase concentration + selected hypsin concentration sTo determine optomum time for each incubation