Glycosylation

a. What is O-linked glycosylation and how do they differ from N-linked
b. Cite an example of how bacterial organisms can sometimes mimic O-linked
glycosylation to confuse mammalian host cells. (brief description)
i. Focus on one bacterial organism? Virulent or commensal.
ii. What mammalian host cell do they typically influence?
iii. O-linked branched chains and Mechanism of action
Remember to Cite any references (if any)

  1. Antibody-drug conjugates are the most recent advancement in current
    a. Brief explanation of what these are?
    b. Provide an example: either currently being researched or on the market
    c. How are these conjugated to the drug – counterparts? What kind of bonds are
    used? (Brief explanation no more than a page)
    d. Provide a Schematic or Diagram of a process chain with a brief explanation for
    each process within the chain.
    For both a and b please cite references (if any)
  2. The expression of Rv2629 modulates the growth of M. smegmatis under aerobic
    conditions. The growth curve of the recombinant strains exhibits reduced expression or
    in some cases overexpression, which is influencing the growth rates of specific strains.
    The cell growth is being measured using turbidity (optical density counts) at 600 nm
    absorbance values. The abbreviations were: MS (wild type), MSP (control strain transformed with the pMV261
    plasmid), and MSpACT (control strain transformed with the pACT plasmid). The results indicated (A)
    comparatively
    slow growth of MSW. (B) The comparatively rapid growth of MSL;
    Reference: Dan Liu et al Front. Microbiol., 15 November 2017, https://doi.org/10.3389/fmicb.2017.02231
    a. From the graph – interpret which 3 growth phases are being studied here.
    b. Which phase is being influenced the most?
    c. State 3 reasons for why this particular phase is important.
  3. What are some important characteristics of an appropriate host cell line for bioprocess design
    for monoclonal antibody production for the treatment of cancer?
  4. How do enzymes influence the reaction energy in a catalysis process and what are some key
    differences between rapid -equilibrium versus quasi-steady state assumptions of enzyme kinetics?
  5. Which metabolic pathway control mechanism plays a major role in the regulation of the ProlineArginine
    (KEGG) pathway? Briefly discuss this specific metabolic control mechanism.
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