Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-mass spectrometry (LC-MS(/MS)) instruments ( assignments)

Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-mass spectrometry (LC-MS(/MS)) instruments ( assignments)- Please who will work in this paper must be have knowledge in GC-MS and LC-MS and Forensic toxicology. - language must be simple and clear without complicated words. - answer 11 questions with basics. - any answers please provide references use in pdf or web site. - Any questions will be welcomed before finish the paper to check the answer that done. - check the word I will uploaded and I will post the questions here also. The questions are: 1- Briefly describe the ionisation mechanisms for ESI, APCI and APPI. - Describe with 150 words for each ( ESI, APCI and APPI). Total 450 words and separated in 3 paragraphs. 2- Briefly describe how the following mass analysers filter / separate ions based on their mass-to-charge ratio; ion trap, quadrupole and time-of-flight? - Describe it in 275 words for the three type please. 3- Explain what isocratic and gradient elution is, include when you may need to use either type of elution and comment on their benefits? - Explain in 220 words please. 4- Compare reversed phase versus normal chromatography and highlight the main differences between the two types of chromatographic separation? - Explain in 220 words please. 5- Draw a fully labelled schematic EI ion source diagram? - Draw 6- Briefly describe the ionisation mechanisms for both EI and CI ? - 120 words for each EI and CI ( total 240) 7- Explain the importance of air/water checks and tunes in GC/MS ? - explain in 150 words please. 8- For both positive and negative ionisation give at least two examples of analyte precursor ion adducts that can be formed e.g. [M+?]+ - 100 words. 9- In addition to the notes on your observations during the laboratory, discuss the factors which you have altered and explain the effect they have had on the chromatographic separation? - The factors that has been altered ( oven temperature, temperature program ramp and inlet mode (splitless/ split). 150 words please 10- Based on its mass spectrum, discuss the use of diazepam-d5 as an internal standard in the analysis of diazepam? - 50 words. 11- Explain why morphine has to be derivatised prior to analysis by GC/MS. - 75 words.