Detection and analysis of recombinant dihydrofolate reductase


Describe the use of recombinant proteins in research, pharmaceuticals, and other
 Identify challenges and considerations for the production of recombinant proteins
 Suggest how expression and purification of tagged dihydrofolate reductase proteins
could be used for further research and drug design
 Analyze fusion proteins computationally through sequence analysis
 Analyze fusion proteins experimentally by Western Blot
 Identify potential sources of error/variation based on experimental procedures and
analysis of your results

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