1.Critically evaluate the chromatograms provided and discuss whether they are ideal or not.
2.From the data provided (via powerpoint) explain, in general terms, what impact a change in the following parameters has on the chromatographic separation of a mixture of compounds. (you can use various examples from the data provided to support the points made)
(i)A change in mobile phase composition
(ii)A change in mobile phase
(iii)A change in mobile phase flow rate
(iv)A change in column length
3.From the data provided (via powerpoint) explain how the efficiency of the chromatography system for ibruporen was altered with a change in the following parameters. (you can use various examples from the data provided to support the points made)
(i)A change in mobile phase composition
(ii)A change in mobile phase
(iii)A change in mobile phase flow rate
(iv)A change in column length
For question 1 focus on the 4 key properties of an ideal chromatogram.
For question 2 focus on all parameters of the resolution equation – retention, selectivity and efficiency
For question 3 focus on efficiency only – HETP and band broadening