Biomedical Science

  HPLC Course Title: Biomedical Science Unit Title: Analytical Techniques in Biomedical Science 1Houre writ up exam to answer this Question (use essay format) QUESTON- Describe the principle of chromatographic separation with special reference to high performance liquid chromatography (HPLC). Your answer should include a description of the component and their function in a typical HPLC system? -Definition *Chromatography *HPLC - Diagram…..Components *Mobil-stationary …..Phases *Affinity *Elution volume *Retention time *Principle of CHR * Resolution *Normal or reverse…..Phases (polar –nonpolar) *Detector *Injection sample (How many types) -How can measure attention time? -Culprit the calm internal STD (standard) -Why we need to measure the standard? - Application of (HPLC) -How to calculate the result?