QUOTE DIRECTLY FROM THE PAPER. You need to use your own words.
Read the attached article: “
A Mitochondrial Pyruvate Carrier Required for Py
ruvate Uptake in Yeast,
Drosophila, and Humans,”
and answer the following questions.
The authors describe experiments performed to identify the carrier protein involved in pyruvate uptake
into the mitochondria.
How did the authors demonstrate that the
Mpc1 protein was located in the mitochondrial inner
2) Describe how
the authors demonstrate
that Mpc1 protein function was conserved through
3) The Drosophila that lacked dMPC1 (dMPC1
) died q
uickly on a sugar only diet (Figure 2A). What does
this suggest about sugar metabolism in these flies?
How did measurement of metabolites in Figure 2F,
3A and 3B help the authors understand why the dMPC1
flies were dying?
4) Even without a pyruvate carri
to transport pyruvate from the cytosol to the matrix, the
a can still have some pyruvate in the matrix.
Describe one way that pyruvate can be
generated in the mitochondrial matrix
in the absence of Mpc1.
Describe the experiment in this pa
per that most directly demonstrates that Mpc1 is a pyruvate
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