Your research project is the characterization of a novel organism: I. confusia. You determine that I. confusia has all the enzymes for glycolysis, can produce lactate as a fermentation product of glucose. It also has a TCA cycle, and an electron transport chain, that uses oxygen as a terminal electron acceptor. I. confusia has a membrane bound ATPase that converts pairs of protons to ATP. I confusia can use group translocation to move glucose across the cytoplasmic membrane.

You are investigating I. confusia’s ability to grow on the (fictional) artificial sweetener biocyonine, so far, you have observed I. confusia can grow aerobically on minimal media where biocyonine is the only carbon and energy source.

I. confusia uses a transporter to move the bicyonine across the cytoplasmic membrane. The transporter moves two molecules of bicyonine at a time.

The cell spends 5 ATP to transport 2 biocyonine molecules.

I. confusia has a pathway that converts each molecule of bicyonine into 1 molecule of citrate.

The citrate can proceed down the remaining of the TCA cycle.

What is the highest number of ATP that will be made by substrate level phosphorylation if I. confusia is consuming two molecules of bicyonine aerobically?

What is the highest number of molecules of NADH that will be made when

I. confusia consumes two molecules of bicyonine aerobically?

What is the highest number of FADH2 that will be made when I. confusia consumes two molecules of bicyonine aerobically?

What is the highest number of pairs of protons will be pumped out of the cell when I. confusia consumes two molecules of bicyonine aerobically?