Phage Lambda Sequence Analysis
- Copy sequence of phage lambda from here and restrict it by using the restriction analysis tool in step 2.
- NEBcutter V2.0 or resources at NCBI.
- Draw a restriction map showing the restriction sites for EcoRI, SmaI, and HaeIII.
Questions: - What are the restriction enzymes, when were they discovered, and who discovered them?
- How many restriction enzymes have been identified?
- How are their names written?
- BamHI, EcoRI, HindIII, and PstI. Search and find the sources of these enzymes. Also, write the recognition sites/sequences for these enzymes. You may make a small table for this answer.
- What is the name of the tool used to measure DNA in volumes less than a milliliter?
- What is gel electrophoresis?
- What type of gels are used for gel electrophoresis?
- What are limitations of gel electrophoresis technique?
- Why type of methods are used to visualize DNA after gel electrophoresis? Write two different chemical methods?
- What evidence do you have that each enzyme cuts the DNA at different locations?
- In gel electrophoresis what is the direction of the movement of DNA?
- Why do we add blue dye to the DNA before loading it on the gel? T
Draw restriction maps of lambda DNA; one for each enzyme separately or one combined for all three enzymes.